GeneTex
United States (US)

IL3 Receptor alpha antibody [N2C2], Internal

Overexpression
GTX101563_42214_20151012_IHC-P_19011506_353.jpg
GTX101563_41983_20151029_WB_19011506_357.jpg
GTX101563_40268_IHC_19011506_875.jpg

Cat No. GTX101563

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

THP-1 , IL3 Receptor alpha-transfected (+) 293T whole cell extracts
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.65mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL3 Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD123 antibody, IL3R antibody, IL3RAY antibody, IL3RX antibody, IL3RY antibody, MGC34174 antibody, hIL-3Ra antibody, IL3RA antibody, CD123 antigen antibody, IL-3R-alpha antibody, IL-3RA antibody, interleukin-3 receptor subunit alpha antibody, IL-3 receptor alpha SP2 isoform antibody, IL-3 receptor subunit alpha antibody, IL-3R subunit alpha antibody, "interleukin 3 receptor, alpha (low affinity) antibody"

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101563_42214_20151012_IHC-P_19011506_353.jpg

GTX101563 IHC-P Image

IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein at cell membrane and cytoplasm in human esophageal cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal cancer.
IL3 Receptor alpha antibody [N2C2], Internal (GTX101563) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101563_41983_20151029_WB_19011506_357.jpg

GTX101563 WB Image

Overexpression

IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein by western blot analysis. THP-1 whole cell extracts (30 μg), non-transfected (-) and IL3 Receptor alpha-transfected (+) 293T whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL3 Receptor alpha antibody (GTX101563) diluted by 1:1000.

GTX101563_40268_IHC_19011506_875.jpg

GTX101563 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL3 Receptor alpha(GTX101563) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169