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IL3 Receptor alpha antibody [N2C2], Internal

Overexpression
Anti-IL3 Receptor alpha antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101563
Anti-IL3 Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX101563
Anti-IL3 Receptor alpha antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101563

Cat No. GTX101563

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all IL3 Receptor alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

THP-1 , IL3 Receptor alpha-transfected (+) 293T whole cell extracts

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL3 Receptor alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 3 receptor subunit alpha , CD123 , IL3R , IL3RAY , IL3RX , IL3RY , hIL-3Ra

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL3 Receptor alpha antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101563

GTX101563 IHC-P Image

IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein at cell membrane and cytoplasm in human esophageal cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal cancer.
IL3 Receptor alpha antibody [N2C2], Internal (GTX101563) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL3 Receptor alpha antibody [N2C2], Internal used in Western Blot (WB). GTX101563

GTX101563 WB Image

Overexpression

IL3 Receptor alpha antibody [N2C2], Internal detects IL3 Receptor alpha protein by western blot analysis. THP-1 whole cell extracts (30 μg), non-transfected (-) and IL3 Receptor alpha-transfected (+) 293T whole cell extracts (5 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IL3 Receptor alpha antibody (GTX101563) diluted by 1:1000.

Anti-IL3 Receptor alpha antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101563

GTX101563 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL3 Receptor alpha(GTX101563) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kuwatsuka S et al. J Dermatol 2018; (Epub) Numerous plasmacytoid dendritic cell infiltration in HIV-associated psoriasis relieved only with antiretroviral therapy.
Application : IHC-P
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169