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IL3 antibody [3B11]

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in Western Blot (WB). GTX84295
Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295
Anti-IL3 antibody [3B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84295

Cat. No. GTX84295

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3B11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human
Package
100 μl ($519)
See all IL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 3 , IL-3 , MCGF , MULTI-CSF

Cellular Localization

Secreted

Background

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Database

Research Area

DATA IMAGES

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human colon tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human breast tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in Western Blot (WB). GTX84295

GTX84295 WB Image

WB analysis of HEK293T cells transfected with IL3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84295 IL3 antibody [3B11].
Loading : 5 ug per lane

Anti-IL3 antibody [3B11] used in IHC (Paraffin sections) (IHC-P). GTX84295

GTX84295 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84295 IL3 antibody [3B11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL3 antibody [3B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84295

GTX84295 ICC/IF Image

ICC/IF analysis of A549 cells using GTX84295 IL3 antibody [3B11].

REFERENCE

Application Reference

EJ Thompson et al. Growth Factors 2024; 223 : 105824 Interleukin-3 production by basal-like breast cancer cells is associated with poor prognosis
Application : IHC-P
L Xue et al. Neural Regen Res 2024; 19 (9) : 2027-2035 Transplantation of human placental chorionic plate-derived mesenchymal stem cells for repair of neurological damage in neonatal hypoxic-ischemic encephalopathy
Application : IHC-Fr
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519