APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2.5 - 5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat Cell Lysate(GTX27899)
Product Note
This antibody detects the largest isoform of IL-32.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
IL-32 antibody was raised against a 16 amino acid synthetic peptide from near the center of human IL-32.The immunogen is located within amino acids 50 - 100 of IL-32.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 32 , IL-32alpha , IL-32beta , IL-32delta , IL-32gamma , NK4 , TAIF , TAIFa , TAIFb , TAIFc , TAIFd
Cellular Localization
Secreted
Background
Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2 . It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32 (2). IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis (3).
Database
Research Area
DATA IMAGES
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GTX85069 WB Image
WB analysis of Jurkat cell lysate using GTX85069 IL32 antibody. Working concentration : (A) 2.5 and (B) 5 μg/ml
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REFERENCE
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REVIEW
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