APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:2000-1:10000 |
| 1:20000-1:100000 |
| 1:1:1000-1:5000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
20mM Potassium Phosphate, 150mM NaCl, 1% BSA
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length recombinant human IL-32A protein.
Purification
IgG fraction
Conjugation
Biotin
Ratio : 1-2 molecules BAC per Goat IgG molecule.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 32 , IL-32alpha , IL-32beta , IL-32delta , IL-32gamma , NK4 , TAIF , TAIFa , TAIFb , TAIFc , TAIFd
Cellular Localization
Secreted
Background
IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32a, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFa and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Database
Research Area
DATA IMAGES
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GTX48653 WB Image
Western blot using GeneTex's HRP conjugated anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant human IL-32A. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.
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REFERENCE
There are currently no references for IL32A antibody (Biotin) (GTX48653). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL32A antibody (Biotin) (GTX48653). Be the first to share your experience with this product.
