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IL33 antibody [Bondy-1-1] (Azide free)

Anti-IL33 antibody [Bondy-1-1] (Azide free) used in ELISA (ELISA). GTX33928-00
Anti-IL33 antibody [Bondy-1-1] (Azide free) used in (). GTX33928-00

Cat. No. GTX33928-00

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Bondy-1-1

Isotype

 IgG2b

Application

 ELISA, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($509)
See all IL33 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Product Note

Recognizes mouse IL-33.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse recombinant IL-33.

Purification

Protein A-affinity purified.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 33 , 9230117N10Rik , Il-33 , Il1f11 , NF-HEV

Cellular Localization

Nucleus , Secreted

Database

Research Area

DATA IMAGES

Anti-IL33 antibody [Bondy-1-1] (Azide free) used in ELISA (ELISA). GTX33928-00

GTX33928-00 ELISA Image

Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free). Methods: IL-33 (mouse) was coated on an ELISA plate at 1μg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 100μl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).

Anti-IL33 antibody [Bondy-1-1] (Azide free) used in (). GTX33928-00

GTX33928-00 Image

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 509