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IL33 antibody [Bondy-1-1] (Azide free)

Anti-IL33 antibody [Bondy-1-1] (Azide free) used in ELISA (ELISA). GTX33928-00
Anti-IL33 antibody [Bondy-1-1] (Azide free) used in  (). GTX33928-00

Cat No. GTX33928-00

Host

Mouse

Clonality

Monoclonal

Clone Name

Bondy-1-1

Isotype

IgG2b

Application

ELISA, Functional Assay

Reactivity

Mouse
Package
100 μg ($449)

APPLICATION

Application Note

Functional Assay: Inhibits the binding of mouse IL-33 to ST2/IL-1RAcP.

Calculated MW

30 kDa. ( Note )

Specificity/Sensitivity

Recognizes mouse IL-33.

PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Mouse recombinant IL-33.

Purification

Protein A-affinity purified.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Interleukin 33 , 9230117N10Rik , Il-33 , Il1F11 , Nf-Hev , Il33

Database

Research Area

DATA IMAGES

Anti-IL33 antibody [Bondy-1-1] (Azide free) used in ELISA (ELISA). GTX33928-00

GTX33928-00 ELISA Image

Binding of IL-33 (mouse) to ST2/IL-1RAcP is inhibited by IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free). Methods: IL-33 (mouse) was coated on an ELISA plate at 1μg/ml. IL-33 (mouse), mAb (rec.) (blocking) (Bondy-1-1) (preservative free) or an unrelated mAb (recombinant) (Control) were added (starting at 40μg/ml with a twofold serial dilution) together with 100μl of supernatant of cells containing ST2 (human):Fc/IL-1RAcP (human):Fc. After incubation for 1h at RT, the binding was detected using an anti-Fc human antibody (HRP).

Anti-IL33 antibody [Bondy-1-1] (Azide free) used in  (). GTX33928-00

GTX33928-00 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

REFERENCE

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REVIEW

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