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IL33 antibody

Anti-IL33 antibody used in IHC (Paraffin sections) (IHC-P). GTX134566
Anti-IL33 antibody used in Western Blot (WB). GTX134566
Anti-IL33 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134566

Cat. No. GTX134566

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IL33 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IL33. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887310

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 33 , C9orf26 , DVS27 , IL1F11 , NF-HEV , NFEHEV

Cellular Localization

Nucleus,Secreted

Background

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-IL33 antibody used in IHC (Paraffin sections) (IHC-P). GTX134566

GTX134566 IHC-P Image

IL33 antibody detects IL33 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
IL33 stained by IL33 antibody (GTX134566) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IL33 antibody used in Western Blot (WB). GTX134566

GTX134566 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with IL33 antibody (GTX134566) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 22307629

Anti-IL33 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134566

GTX134566 ICC/IF Image

IL33 antibody detects IL33 protein at cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL33 stained by IL33 antibody (GTX134566) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169