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IL33 antibody

Anti-IL33 antibody used in Western Blot (WB). GTX31317
Anti-IL33 antibody used in IHC (Paraffin sections) (IHC-P). GTX31317
Anti-IL33 antibody used in Western Blot (WB). GTX31317
Anti-IL33 antibody used in IHC (Paraffin sections) (IHC-P). GTX31317
Anti-IL33 antibody used in Western Blot (WB). GTX31317
Anti-IL33 antibody used in Western Blot (WB). GTX31317

Cat No. GTX31317

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

Human Lymphoid Tissue Tissue Slide(GTX21833) , A20 Cell Lysate(GTX27180)

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the center of human IL-33.The immunogen is located within amino acids 150 - 200 of IL-33.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 33 , C9orf26 , DVS27 , IL1F11 , NF-HEV , NFEHEV

Cellular Localization

Nucleus,Secreted

Background

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-IL33 antibody used in Western Blot (WB). GTX31317

GTX31317 WB Image

WB analysis of A-20 cell lysate using GTX31317 IL33 antibody.
Dilution : 1μg/mL(Lane A) and 2μg/mL(Lane B)
Loading : 15μg of lysates per lane

Anti-IL33 antibody used in IHC (Paraffin sections) (IHC-P). GTX31317

GTX31317 IHC-P Image

IHC-P analysis of 4% PFA-fixed Human Lymph Node using GTX31317 IL33 antibody.
Dilution : 20μg/mL

Anti-IL33 antibody used in Western Blot (WB). GTX31317

GTX31317 WB Image

WB analysis of 5ng (Lane 1) and 25ng (Lane 2) partial human recombinant protein using GTX31317 IL33 antibody. Il-33 partial human recombinant protein: 112-270aa, 17.9kD, 36kD band is the dimer.
Dilution : 1μg/mL

Anti-IL33 antibody used in IHC (Paraffin sections) (IHC-P). GTX31317

GTX31317 IHC-P Image

IHC-P analysis of formaldehyde fixed Human Lymph Node using GTX31317 IL33 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL

Anti-IL33 antibody used in Western Blot (WB). GTX31317

GTX31317 WB Image

WB analysis of Human Lung tissue lysate using GTX31317 IL33 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane

Anti-IL33 antibody used in Western Blot (WB). GTX31317

GTX31317 WB Image

WB analysis of Human Lymph Node tissue lysate using GTX31317 IL33 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339