APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1-2 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lymphoid Tissue Tissue Slide(GTX21833) , A20 Cell Lysate(GTX27180)
Predict Reactivity
Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS with 0.02% sodium azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
IL-33 antibody was raised against a 19 amino acid synthetic peptide from near the center of human IL-33.The immunogen is located within amino acids 150 - 200 of IL-33.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 33 , C9orf26 , DVS27 , IL1F11 , NF-HEV , NFEHEV
Cellular Localization
Nucleus,Secreted
Background
IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]
Database
Research Area
DATA IMAGES
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GTX31317 WB Image
WB analysis of A-20 cell lysate using GTX31317 IL33 antibody.
Dilution : 1μg/mL(Lane A) and 2μg/mL(Lane B)
Loading : 15μg of lysates per lane
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GTX31317 IHC-P Image
IHC-P analysis of 4% PFA-fixed Human Lymph Node using GTX31317 IL33 antibody.
Dilution : 20μg/mL
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GTX31317 WB Image
WB analysis of 5ng (Lane 1) and 25ng (Lane 2) partial human recombinant protein using GTX31317 IL33 antibody. Il-33 partial human recombinant protein: 112-270aa, 17.9kD, 36kD band is the dimer.
Dilution : 1μg/mL
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GTX31317 IHC-P Image
IHC-P analysis of formaldehyde fixed Human Lymph Node using GTX31317 IL33 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL
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GTX31317 WB Image
WB analysis of Human Lung tissue lysate using GTX31317 IL33 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane
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GTX31317 WB Image
WB analysis of Human Lymph Node tissue lysate using GTX31317 IL33 antibody.
Dilution : 2μg/mL
Loading : 15μg of lysates per lane
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REFERENCE
There are currently no references for IL33 antibody (GTX31317). Be the first to share your publications with this product.
REVIEW
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