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IL4 antibody [25D2] (Biotin)

Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759
Anti-IL4 antibody [25D2] (Biotin) used in ELISA (ELISA). GTX15759

Cat. No. GTX15759

Host

Rat

Clonality

Monoclonal

Clone Name

25D2

Isotype

IgG1

Application

WB, ICC/IF, FACS, ELISA, IHC, Neutralizing/Inhibition

Reactivity

Human
Package
250 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
FACS Assay dependent
ELISA 0.006-25 μg/mL
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.96 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-4 (CHO cell-derived)

Purification

Protein G purified

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 4 , BCGF-1 , BCGF1 , BSF-1 , BSF1 , IL-4

Cellular Localization

Secreted

Background

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759

GTX15759 WB Image

WB analysis of 1 ug of human recombiannt IL-4 protein in non-reducing sample buffer using GTX15759 IL4 antibody [25D2].
Dilution : 1:1000

Anti-IL4 antibody [25D2] (Biotin) used in ELISA (ELISA). GTX15759

GTX15759 ELISA Image

ELISA analysis of 1 μg/mL human IL-4 recombinant protein diluted in carbonate/bicarbonate buffer using serial diluted GTX15760 IL4 antibody [25D2].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399