APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| Assay dependent |
| Assay dependent |
| 0.006-25 μg/mL |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.96 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-4 (CHO cell-derived)
Purification
Protein G purified
Conjugation
Biotin
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 4 , BCGF-1 , BCGF1 , BSF-1 , BSF1 , IL-4
Cellular Localization
Secreted
Background
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759](/templates/default/js/lightbox/images/loading.gif "Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759")
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GTX15759 WB Image
WB analysis of 1 ug of human recombiannt IL-4 protein in non-reducing sample buffer using GTX15759 IL4 antibody [25D2].
Dilution : 1:1000
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GTX15759 ELISA Image
ELISA analysis of 1 μg/mL human IL-4 recombinant protein diluted in carbonate/bicarbonate buffer using serial diluted GTX15760 IL4 antibody [25D2].
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REFERENCE
There are currently no references for IL4 antibody [25D2] (Biotin) (GTX15759). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL4 antibody [25D2] (Biotin) (GTX15759). Be the first to share your experience with this product.
![Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759](/upload/website/prouct_img/small/GTX15759/GTX15759_1526_WB_19011519_127.jpg "Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759")
![Anti-IL4 antibody [25D2] (Biotin) used in ELISA (ELISA). GTX15759](/upload/website/prouct_img/small/GTX15759/GTX15759_62_ELISA_19011519_645.jpg "Anti-IL4 antibody [25D2] (Biotin) used in ELISA (ELISA). GTX15759")
![Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759](/upload/website/prouct_img/normal/GTX15759/GTX15759_1526_WB_19011519_127.jpg "Anti-IL4 antibody [25D2] (Biotin) used in Western Blot (WB). GTX15759")
![Anti-IL4 antibody [25D2] (Biotin) used in ELISA (ELISA). GTX15759](/upload/website/prouct_img/normal/GTX15759/GTX15759_62_ELISA_19011519_645.jpg "Anti-IL4 antibody [25D2] (Biotin) used in ELISA (ELISA). GTX15759")