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IL4 antibody [GT1225]

Recombinant antibody
Anti-IL4 antibody [GT1225] used in Western Blot (WB). GTX02822
Anti-IL4 antibody [GT1225] used in Western Blot (WB). GTX02822
Anti-IL4 antibody [GT1225] used in Western Blot (WB). GTX02822

Cat. No. GTX02822

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1225

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($419)
See all IL4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human IL4

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 4 , BCGF-1 , BCGF1 , BSF-1 , BSF1 , IL-4

Cellular Localization

Secreted

Background

The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL4 antibody [GT1225] used in Western Blot (WB). GTX02822

GTX02822 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with IL4 antibody [GT1225] (GTX02822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL4 antibody [GT1225] used in Western Blot (WB). GTX02822

GTX02822 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL4 antibody [GT1225] (GTX02822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL4 antibody [GT1225] used in Western Blot (WB). GTX02822

GTX02822 WB Image

WB analysis of various samples using GTX02822 IL4 antibody [GT1225].
Dilution : 1:1000
Loading : 25μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 419