APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02993, Detection: GTX02994-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native and recombinant human IL-4.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-4
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCGF1,BSF1,IL4,interleukin 4,Interleukin 4
Cellular Localization
Secreted
Background
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-IL4 antibody [IL4-II] (Biotin) used in ELISA (ELISA). GTX02994-02](/templates/default/js/lightbox/images/loading.gif "Anti-IL4 antibody [IL4-II] (Biotin) used in ELISA (ELISA). GTX02994-02")
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GTX02994-02 ELISA Image
Sandwich ELISA analysis of human IL-4 protein using GTX02993 IL4 antibody [IL4-I] as coating antibody and GTX02994-02 IL4 antibody [IL4-II] (Biotin) as detecting antibody.
Substrate : pNPP
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REFERENCE
There are currently no references for IL4 antibody [IL4-II] (Biotin) (GTX02994-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL4 antibody [IL4-II] (Biotin) (GTX02994-02). Be the first to share your experience with this product.
![Anti-IL4 antibody [IL4-II] (Biotin) used in ELISA (ELISA). GTX02994-02](/upload/website/prouct_img/small/GTX02994-02/GTX02994-02_20210507_ELISA_w_23053123_467.webp "Anti-IL4 antibody [IL4-II] (Biotin) used in ELISA (ELISA). GTX02994-02")
![Anti-IL4 antibody [IL4-II] (Biotin) used in ELISA (ELISA). GTX02994-02](/upload/website/prouct_img/normal/GTX02994-02/GTX02994-02_20210507_ELISA_w_23053123_467.webp "Anti-IL4 antibody [IL4-II] (Biotin) used in ELISA (ELISA). GTX02994-02")