PRODUCT
Summary
The MP4-25D2 antibody is specific for human IL-4, a pleiotropic cytokine that Th2 cell differentiation, B cell proliferation and differentiation, and B cell class switching to IgE. MP4-25D2 is a neutralizing antibody and is reported to cross react with rhesus monkey IL-4.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.0375 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄ (pH7.2), 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.0075 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Allophycocyanin (APC)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 4 , BCGF1 , BSF1 , IL4 , Interleukin 4
Cellular Localization
Secreted
Background
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07](/templates/default/js/lightbox/images/loading.gif "Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07")
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GTX01492-07 FACS Image
FACS analysis of human peripheral blood lymphocytes (with PMA and Ionomycin stimulation) using GTX01492-07 IL4 antibody [MP4-25D2] (APC).
Right panel : co-stained with IL4 antibody [MP4-25D2] (APC) and Human CD3 antibody (PE)
Left panel : co-stained with isotype control and Human CD3 antibody (PE)
antibody amount : 0.0375 μg (5 μl)
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REFERENCE
REVIEW
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![Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07](/upload/website/prouct_img/small/GTX01492-07/GTX01492-07_20200428_FACS34_20042900_969.jpg "Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07")
![Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07](/upload/website/prouct_img/normal/GTX01492-07/GTX01492-07_20200428_FACS34_20042900_969.jpg "Anti-IL4 antibody [MP4-25D2] (APC) used in Flow cytometry (FACS). GTX01492-07")