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IL4 antibody

Cat. No. GTX10755

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC, Neutralizing/Inhibition

Reactivity

Human
Package
25 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/ml
IHC 0.5-5 μg/ml
Neutralizing/Inhibition 0.02-0.1 μg/ml
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Product Note

The antibody will neutralize the biological activity of recombinant human IL-4, but not recombinant mouse IL-4.

PROPERTIES

Form

Liquid

Buffer

PBS, 5% Trehalose

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

recombinant human IL-4.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 4 , BCGF-1 , BCGF1 , BSF-1 , BSF1 , IL-4

Cellular Localization

Secreted

Background

Interleukin 4 (IL4) is a multifunctional lymphokine which interacts with cells of multilineages including T cells, B cells, thymocytes, hematopoietic cells and fibroblasts. IL4 was first described as stimulating B-lymphocyte proliferation in the presence of anti-IgM antibodies. It was then shown that IL4 could induce the expression of molecules of the class II MHC in resting B cells. Interleukin 4 is a complex glycoprotein released by a subset of activated T cells. The molecular weight of IL4 occurring naturally is approximately 12 - 20 kD.

Database

Research Area

REFERENCE

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REVIEW

IL4 antibody Cat. No. GTX10755

Rating ( Average 5 based on 1 users reviews)
Application
Date : Anonymous submitted on 07-Mar-2022
Score :
Sample : Huma skin tissue specimen
Blocking : 0.3% H2O2 meta, RT, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate buffer (ph 6.0)
Primary Antibody : 1:200, 4°C, > 12Hr
Lane Description : Generalized erythroderma.
SDS
PBS.pdf
Package List Price ($)
$ 399