APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02997, Detection: GTX02995-02 / GTX02996-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native and recombinant human IL-5.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-5
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
EDF,IL5,TRF,interleukin 5
Cellular Localization
Secreted
Background
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02](/templates/default/js/lightbox/images/loading.gif "Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02")
|
GTX02995-02 ELISA Image
Sandwich ELISA analysis of human IL-5 protein using GTX02997 IL5 antibody [TRFK5] as coating antibody and GTX02995-02 IL5 antibody [5A10] (Biotin) as detecting antibody.
Substrate : pNPP
|
REFERENCE
There are currently no references for IL5 antibody [5A10] (Biotin) (GTX02995-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL5 antibody [5A10] (Biotin) (GTX02995-02). Be the first to share your experience with this product.
![Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02](/upload/website/prouct_img/small/GTX02995-02/GTX02995-02_20210507_ELISA_21050619_617.jpg "Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02")
![Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02](/upload/website/prouct_img/normal/GTX02995-02/GTX02995-02_20210507_ELISA_21050619_617.jpg "Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02")