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IL5 antibody [5A10] (Biotin)

Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02

Cat. No. GTX02995-02

Host

 Rat

Clonality

 Monoclonal

Clone Name

 5A10

Isotype

 IgG2a

Application

 ELISA, Sandwich ELISA

Reactivity

 Human, Monkey
Package
250 μg ($399)
See all IL5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX02997, Detection: GTX02995-02 / GTX02996-02.
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant human IL-5.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-5

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

EDF,IL5,TRF,interleukin 5

Cellular Localization

Secreted

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02

GTX02995-02 ELISA Image

Sandwich ELISA analysis of human IL-5 protein using GTX02997 IL5 antibody [TRFK5] as coating antibody and GTX02995-02 IL5 antibody [5A10] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 399