Indirect ELISA analysis was performed by coating the plate with recombinant Human IL5 protein, His tag (GTX139317-pro) (200-3.13 nM). Coated protein was probed with IL5 antibody [GT30] (GTX641122) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.