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IL5 antibody [R013]

Anti-IL5 antibody [R013] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX02158

Cat No. GTX02158

Host

Rabbit

Clonality

Monoclonal

Clone Name

R013

Isotype

IgG

Application

Neutralizing/Inhibition

Reactivity

Human
Package
200 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
1. In a functional ELISA which immobilized Human IL5 protein at 0.4 μg/ml (100 μl/well) in the plate, this antibody can block the binding of 2 μg/ml Human IL5R protein to Human IL5 protein. And the EC₅₀ is 0.23 μg/ml.
2. The neutralization activity of this antibody is measured by its ability to neutralize IL5-induced proliferation in TF-1 cells. The Neutralization Dose (ND₅₀) is typically 2-8 ng/ml in the presence of 1.25 ng/ml Human IL5 protein.

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered Histidine and Arginine buffer (pH6.0), 120mM NaCl, 0.02% Tween-80

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human IL-5 / IL5 / Interleukin 5 protein

Purification

Purified by Protein A

Endotoxin

< 3 EU/mg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 5 , EDF , IL-5 , TRF

Cellular Localization

Secreted

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-IL5 antibody [R013] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX02158

GTX02158 Neutralizing/Inhibition Image

Proliferation of TF-1 cells elicited by recombinant human IL5 (1.25 ng/ml) is neutralized by increasing concentrations of GTX02158 IL5 antibody [R013].
ND₅₀ : 2-8 ng/ml.

REFERENCE

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REVIEW

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Package List Price ($)
$ 329