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IL5 antibody [R013]

Anti-IL5 antibody [R013] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX02158

Cat. No. GTX02158

Host

Rabbit

Clonality

Monoclonal

Clone Name

R013

Isotype

IgG

Application

Neutralizing/Inhibition

Reactivity

Human
Package
200 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
Neutralizing/Inhibition Assay dependent

Note :

Neutralizing/Inhibition
1. In a functional ELISA which immobilized Human IL5 protein at 0.4 μg/ml (100 μl/well) in the plate, this antibody can block the binding of 2 μg/ml Human IL5R protein to Human IL5 protein. And the EC₅₀ is 0.23 μg/ml.
2. The neutralization activity of this antibody is measured by its ability to neutralize IL5-induced proliferation in TF-1 cells. The Neutralization Dose (ND₅₀) is typically 2-8 ng/ml in the presence of 1.25 ng/ml Human IL5 protein.

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered Histidine and Arginine buffer (pH6.0), 120mM NaCl, 0.02% Tween-80

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human IL-5 / IL5 / Interleukin 5 protein

Purification

Purified by Protein A

Endotoxin

< 3 EU/mg

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 5 , EDF , IL-5 , TRF

Cellular Localization

Secreted

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-IL5 antibody [R013] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX02158

GTX02158 Neutralizing/Inhibition Image

Proliferation of TF-1 cells elicited by recombinant human IL5 (1.25 ng/ml) is neutralized by increasing concentrations of GTX02158 IL5 antibody [R013].
ND₅₀ : 2-8 ng/ml.

REFERENCE

There are currently no references for IL5 antibody [R013] (GTX02158). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399