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IL5 antibody

Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678
Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678
Anti-IL5 antibody used in Western Blot (WB). GTX55678

Cat No. GTX55678

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887934

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 5 , EDF , IL-5 , TRF

Cellular Localization

Secreted

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678

GTX55678 IHC-P Image

IHC-P analysis of human liver tissue using GTX55678 IL5 antibody.
Dilution : 1:100

Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678

GTX55678 IHC-P Image

IHC-P analysis of human stomach tissue using GTX55678 IL5 antibody.
Dilution : 1:100

Anti-IL5 antibody used in Western Blot (WB). GTX55678

GTX55678 WB Image

WB analysis of various sample lysates using GTX55678 IL5 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

REVIEW

IL5 antibody Cat No. GTX55678

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 07-Mar-2022
Score :
Application Tested : IHC-P
Sample : Huma skin tissue specimen
Blocking : 0.3% H2O2 meta, RT, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate buffer (ph 6.0)
Primary Antibody : Undiluted, 4°C, > 12Hr
Lane Description : Generalized erythroderma.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339