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IL5 antibody

Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678
Anti-IL5 antibody used in Western Blot (WB). GTX55678
Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678

Cat. No. GTX55678

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IL5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887934

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 5 , EDF , IL-5 , TRF

Cellular Localization

Secreted

Background

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678

GTX55678 IHC-P Image

IHC-P analysis of human stomach tissue using GTX55678 IL5 antibody.
Dilution : 1:100

Anti-IL5 antibody used in Western Blot (WB). GTX55678

GTX55678 WB Image

WB analysis of various sample lysates using GTX55678 IL5 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678

GTX55678 IHC-P Image

IHC-P analysis of human liver tissue using GTX55678 IL5 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Huriyati E et al. Open Access Maced J Med Sci 2019; 7 (10) : 1635-1640 Association of Inflammation Mediator in Mucosal and Tissue of Chronic Rhinosinusitis with Recurrent Nasal Polyp.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

IL5 antibody Cat. No. GTX55678

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-IL5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55678
Date : Anonymous submitted on 07-Mar-2022
Score :
Application Tested : IHC-P
Sample : Huma skin tissue specimen
Blocking : 0.3% H2O2 meta, RT, 0.5Hr
Fixation : 10% formalin
Permeabilization /Antigen retrieval : Citrate buffer (ph 6.0)
Primary Antibody : Undiluted, 4°C, > 12Hr
Lane Description : Generalized erythroderma.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399