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IL6 antibody [32C11] (Biotin)

Anti-IL6 antibody [32C11] (Biotin) used in ELISA (ELISA). GTX03000-02

Cat. No. GTX03000-02

Host

 Rat

Clonality

 Monoclonal

Clone Name

 32C11

Isotype

 IgG2a

Application

 ELISA, Sandwich ELISA

Reactivity

 Mouse
Package
250 μg ($399)
See all IL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX03002, Detection: GTX03000-02.
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Product Note

This antibody is able to detect native and recombinant mouse IL-6.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse IL-6

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted

Background

This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody [32C11] (Biotin) used in ELISA (ELISA). GTX03000-02

GTX03000-02 ELISA Image

Sandwich ELISA analysis of mouse IL-6 protein using GTX03002 IL6 antibody [MT26B2] as coating antibody and GTX03000-02 IL6 antibody [32C11] (Biotin) as detecting antibody.
Substrate : pNPP

REFERENCE

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REVIEW

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Package List Price ($)
$ 399