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IL6 antibody [3G9]

Anti-IL6 antibody [3G9] used in IHC (Paraffin sections) (IHC-P). GTX84291
Anti-IL6 antibody [3G9] used in Western Blot (WB). GTX84291
Anti-IL6 antibody [3G9] used in Western Blot (WB). GTX84291
Anti-IL6 antibody [3G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84291

Cat. No. GTX84291

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G9

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($539)
See all IL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:50000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.

Database

Research Area

DATA IMAGES

Anti-IL6 antibody [3G9] used in IHC (Paraffin sections) (IHC-P). GTX84291

GTX84291 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84291 IL6 antibody [3G9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-IL6 antibody [3G9] used in Western Blot (WB). GTX84291

GTX84291 WB Image

WB analysis of recombinant human IL-6 using GTX84291 IL6 antibody [3G9].
Dilution : 1:500000

Anti-IL6 antibody [3G9] used in Western Blot (WB). GTX84291

GTX84291 WB Image

WB analysis of HEK293T cells transfected with IL6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84291 IL6 antibody [3G9].
Loading : 5 ug per lane

Anti-IL6 antibody [3G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84291

GTX84291 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with IL6 plasmid using GTX84291 IL6 antibody [3G9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539