APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| 1:100 |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX15762, Detection : GTX15761
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.01 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-6.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6
Cellular Localization
Secreted
Background
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/templates/default/js/lightbox/images/loading.gif "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
|
GTX15762 FACS Image
FACS analysis of permeabilized, PHA-M stimulated THP-1 cells using GTX15762 IL6 antibody [5IL6].
Fixation : 4% formaldehyde
Dilution : 1:100 dilution with IC permeabilization buffer for 30 min on ice
|
|
|
GTX15762 FACS Image
FACS analysis of permeabilized, LPS treated THP-1 cells using GTX15762 IL6 antibody [5IL6].
Fixation : 4% formaldehyde
Dilution : 1:100 with IC permeabilization buffer for 30 min on ice
|
|
|
GTX15762 FACS Image
FACS analysis of permeabilized, LPS treated THP-1 cells using GTX15762 IL6 antibody [5IL6].
Fixation : 4% formaldehyde
Dilution : 1:100 with IC permeabilization buffer for 30 min on ice
|
|
|
GTX15762 ELISA Image
Sandwich ELISA analysis of serial diluted human IL-6 recombinant protein(50ul per well) using IL6 antibody
Coating : GTX15762 IL6 antibody [5IL6] (4 μg/mL)
Detection : GTX15761 IL6 antibody [7IL6] (Biotin) (0.035 μg/mL)
|
REFERENCE
There are currently no references for IL6 antibody [5IL6] (GTX15762). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL6 antibody [5IL6] (GTX15762). Be the first to share your experience with this product.
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/upload/website/prouct_img/small/GTX15762/GTX15762_110_FACS_w_23060620_695.webp "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/upload/website/prouct_img/small/GTX15762/GTX15762_108_FACS_w_23060620_952.webp "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/upload/website/prouct_img/small/GTX15762/GTX15762_109_FACS_w_23060620_402.webp "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
![Anti-IL6 antibody [5IL6] used in ELISA (ELISA). GTX15762](/upload/website/prouct_img/small/GTX15762/GTX15762_64_ELISA_w_23060620_468.webp "Anti-IL6 antibody [5IL6] used in ELISA (ELISA). GTX15762")
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/upload/website/prouct_img/normal/GTX15762/GTX15762_110_FACS_w_23060620_695.webp "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/upload/website/prouct_img/normal/GTX15762/GTX15762_108_FACS_w_23060620_952.webp "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
![Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762](/upload/website/prouct_img/normal/GTX15762/GTX15762_109_FACS_w_23060620_402.webp "Anti-IL6 antibody [5IL6] used in Flow cytometry (FACS). GTX15762")
![Anti-IL6 antibody [5IL6] used in ELISA (ELISA). GTX15762](/upload/website/prouct_img/normal/GTX15762/GTX15762_64_ELISA_w_23060620_468.webp "Anti-IL6 antibody [5IL6] used in ELISA (ELISA). GTX15762")