GeneTex
United States (US)

IL6 antibody [5IL6]

Orthogonal
GTX15762_109_FACS_19011519_471.jpg
GTX15762_108_FACS_19011519_742.jpg
GTX15762_110_FACS_19011519_360.jpg
GTX15762_64_ELISA_19011519_718.jpg

Cat No. GTX15762

Host Mouse
Clonality Monoclonal
Clone Name 5IL6
Isotype IgG1
Application WB, ICC/IF, FACS, ELISA, sELISA
Reactivity Human, Mouse, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS 1:100
ELISA Assay dependent
sELISA Assay dependent

Note :

sELISA
Capture : GTX15762, Detection : GTX15761

Not tested in other applications.

Calculated MW

24 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-6.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 6,Bsf-2,Bsf2,Cdf,Hgf,Hsf,Ifn-Beta-2,Ifnb2,Il-6,Il6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES
GTX15762_109_FACS_19011519_471.jpg

GTX15762 FACS Image

FACS analysis of permeabilized, LPS treated THP-1 cells using GTX15762 IL6 antibody [5IL6].
Fixation : 4% formaldehyde
Dilution : 1:100 with IC permeabilization buffer for 30 min on ice

GTX15762_108_FACS_19011519_742.jpg

GTX15762 FACS Image

FACS analysis of permeabilized, LPS treated THP-1 cells using GTX15762 IL6 antibody [5IL6].
Fixation : 4% formaldehyde
Dilution : 1:100 with IC permeabilization buffer for 30 min on ice

GTX15762_110_FACS_19011519_360.jpg

GTX15762 FACS Image

Orthogonal

FACS analysis of permeabilized, PHA-M stimulated THP-1 cells using GTX15762 IL6 antibody [5IL6].
Fixation : 4% formaldehyde
Dilution : 1:100 dilution with IC permeabilization buffer for 30 min on ice

GTX15762_64_ELISA_19011519_718.jpg

GTX15762 ELISA Image

Sandwich ELISA analysis of serial diluted human IL-6 recombinant protein(50ul per well) using IL6 antibody
Coating : GTX15762 IL6 antibody [5IL6] (4 µg/mL)
Detection : GTX15761 IL6 antibody [7IL6] (Biotin) (0.035 µg/mL)

SDS
PBS.pdf
Package List Price ($)
$ 319