APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1H6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-8 protein.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 6 , BSF2 , CDF , HGF , HSF , IFNB2 , IFNbeta2 , IL6 , Interleukin 6
Cellular Localization
Secreted
Background
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
![Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562](/templates/default/js/lightbox/images/loading.gif "Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562")
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GTX57562 WB Image
WB analysis of various samples using GTX57562 IL6 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Raw264.7 whole cell lysate
Lane 4 : PC-3 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : MCF-7 whole cell lysate
Lane 8 : K562 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57562 FACS Image
FACS analysis of HeLa cells using GTX57562 IL6 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
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GTX57562 FACS Image
FACS analysis of LNCaP cells using GTX57562 IL6 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
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GTX57562 ICC/IF Image
ICC/IF analysis of Hep3B cells using GTX57562 IL6 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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REFERENCE
There are currently no references for IL6 antibody [AT1H6] (GTX57562). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL6 antibody [AT1H6] (GTX57562). Be the first to share your experience with this product.
![Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562](/upload/website/prouct_img/small/GTX57562/GTX57562_20200204_WB_w_23061123_155.webp "Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562](/upload/website/prouct_img/small/GTX57562/GTX57562_20200204_FC2_w_23061123_648.webp "Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562](/upload/website/prouct_img/small/GTX57562/GTX57562_20200204_FC1_w_23061123_375.webp "Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57562](/upload/website/prouct_img/small/GTX57562/GTX57562_20200204_IF3_w_23061123_524.webp "Anti-IL6 antibody [AT1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562](/upload/website/prouct_img/normal/GTX57562/GTX57562_20200204_WB_w_23061123_155.webp "Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562](/upload/website/prouct_img/normal/GTX57562/GTX57562_20200204_FC2_w_23061123_648.webp "Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562](/upload/website/prouct_img/normal/GTX57562/GTX57562_20200204_FC1_w_23061123_375.webp "Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562")
![Anti-IL6 antibody [AT1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57562](/upload/website/prouct_img/normal/GTX57562/GTX57562_20200204_IF3_w_23061123_524.webp "Anti-IL6 antibody [AT1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57562")