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IL6 antibody [AT1H6]

Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562
Anti-IL6 antibody [AT1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57562
Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562
Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562

Cat No. GTX57562

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1H6

Isotype

IgG2a

Application

WB, ICC/IF, FACS

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4), 0.02% sodium azide, 10% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT1H6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL-8 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF2 , CDF , HGF , HSF , IFNB2 , IFNbeta2 , IL6 , Interleukin 6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody [AT1H6] used in Western Blot (WB). GTX57562

GTX57562 WB Image

WB analysis of various samples using GTX57562 IL6 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Raw264.7 whole cell lysate
Lane 4 : PC-3 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : MCF-7 whole cell lysate
Lane 8 : K562 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-IL6 antibody [AT1H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57562

GTX57562 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57562 IL6 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562

GTX57562 FACS Image

FACS analysis of LNCaP cells using GTX57562 IL6 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

Anti-IL6 antibody [AT1H6] used in Flow cytometry (FACS). GTX57562

GTX57562 FACS Image

FACS analysis of HeLa cells using GTX57562 IL6 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

There are currently no references for IL6 antibody [AT1H6] (GTX57562). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for IL6 antibody [AT1H6] (GTX57562). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf