APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture : GTX636571, Detection : GTX636473.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human IL6 Recombinant protein
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2910007
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6
Cellular Localization
Secreted
Background
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Database
Research Area
DATA IMAGES
![Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571](/templates/default/js/lightbox/images/loading.gif "Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571")
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GTX636571 ELISA Image
Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below. Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL) Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)
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GTX636571 ICC/IF Image
IL6 antibody [GT1535] detects IL6 protein at mitochondria and cytoplasm by immunofluorescent analysis.
Sample: SiHa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody [GT1535] (GTX636571) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
There are currently no references for IL6 antibody [GT1535] (GTX636571). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL6 antibody [GT1535] (GTX636571). Be the first to share your experience with this product.
![Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571](/upload/website/prouct_img/small/GTX636571/GTX636571_44566_20220225_ELISA_PAIR_22030201_778.jpg "Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571")
![Anti-IL6 antibody [GT1535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636571](/upload/website/prouct_img/small/GTX636571/GTX636571_44566_20220429_ICC_IF_22052401_615.jpg "Anti-IL6 antibody [GT1535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636571")
![Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571](/upload/website/prouct_img/normal/GTX636571/GTX636571_44566_20220225_ELISA_PAIR_22030201_778.jpg "Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571")
![Anti-IL6 antibody [GT1535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636571](/upload/website/prouct_img/normal/GTX636571/GTX636571_44566_20220429_ICC_IF_22052401_615.jpg "Anti-IL6 antibody [GT1535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636571")