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IL6 antibody [GT1535]

Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571
Anti-IL6 antibody [GT1535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636571

Cat. No. GTX636571

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1535

Isotype

IgG2b

Application

ICC/IF, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX636571, Detection : GTX636473.

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IL6 Recombinant protein

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2910007

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody [GT1535] used in ELISA (ELISA). GTX636571

GTX636571 ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL1168] (GTX636473) (1 μg/mL)

Anti-IL6 antibody [GT1535] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636571

GTX636571 ICC/IF Image

IL6 antibody [GT1535] detects IL6 protein at mitochondria and cytoplasm by immunofluorescent analysis.
Sample: SiHa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IL6 stained by IL6 antibody [GT1535] (GTX636571) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for IL6 antibody [GT1535] (GTX636571). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations