IL6 antibody [HL2389]

Cat. No. GTX638598

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2389
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all IL6 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human IL6.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Gene ID: 3569 IL6
UniProt: P05231 IL6

Research Area

DATA IMAGES

GTX638598 WB Image

Recombinant IL6 were separated by 15% SDS-PAGE, and the membranes were blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX638598 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX638598 ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT1535] (GTX636571) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

GTX638598 IHC-P Image

IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638598 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (2634.91-41.32 pM). Coated protein was probed with IL6 antibody [HL2389] (GTX638598) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

GTX638598 IHC-P Image

IL6 antibody [HL2389] detects IL6 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
IL6 stained by IL6 antibody [HL2389] (GTX638598) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638598 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with IL6 antibody [HL2389] (GTX638598) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX638598 ELISA Image

Sandwich ELISA detection of recombinant Human IL6 protein, His tag (GTX135982-pro) using antibodies as below.

Capture: IL6 antibody [GT519-1] (GTX636573) (5 μg/mL)
Detection: IL6 antibody [HL2389] (GTX638598) (1 μg/mL)

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

IL6 antibody [HL2389]

Cat. No. GTX638598

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX638598 WB Image

GTX638598 WB Image

GTX638598 ELISA Image

GTX638598 IHC-P Image

GTX638598 ELISA Image

GTX638598 IHC-P Image

GTX638598 WB Image

GTX638598 ELISA Image

REFERENCE

REVIEW

Product

Research

Support

Company

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