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IL6 antibody

Anti-IL6 antibody used in ELISA (ELISA). GTX135982

Cat No. GTX135982

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ELISA

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human IL6 recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in ELISA (ELISA). GTX135982

GTX135982 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human IL6 protein, His tag (GTX135982-pro) (1000-15.625 ng/mL). Coated protein was probed with IL-6 antibody (GTX135982) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

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REVIEW

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