*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:2000
|1:400 - 1:800
|1:1000 - 1:5000
|1:400 - 1:800
Not tested in other applications.
Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
77 mg/ml (Please refer to the vial label for the specific concentration.)
This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important asIL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.