GeneTex
  • Country / Location Selection

United States (US)

IL6 antibody

Anti-IL6 antibody used in Western Blot (WB). GTX26672

Cat No. GTX26672

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, IHC

Reactivity

Human, Rat
Package
200 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
IP 1:400 - 1:800
ELISA 1:1000 - 1:5000
IHC 1:400 - 1:800
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Product Note

Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.

Purification

Antiserum

Conjugation

Unconjugated

RRID

AB_368308

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6 , BSF-2 , BSF2 , CDF , HGF , HSF , IFN-beta-2 , IFNB2 , IL-6

Cellular Localization

Secreted

Background

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important asIL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in Western Blot (WB). GTX26672

GTX26672 WB Image

Western blot using GeneTex anti-IL6 antibody (GTX26672).~1 μg of our control protein recombinant human IL-6 was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. The blot shows detection of a band ~21 kDa in size corresponding to anti-IL6 antibody. Molecular weight markers are also shown (MW). After transfer, the membrane was blocked for 30 minutes with 1% BSA-TBST. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with chemiluminescent substrate.

REFERENCE

REVIEW

There are currently no reviews for IL6 antibody (GTX26672). Be the first to share your experience with this product.