Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.1-0.2μg/ml |
0.5-2μg/ml |
Assay dependent |
Note :
Neutralizing/Inhibition
Rabbit anti Mouse Interleukin-6 antibody neutralizes murine IL-6 activity with an ND50 for mIL-6 (3 pgml-1 of between 0.24 and 0.36μg ml-1.
|
Not tested in other applications.
Calculated MW
Product Note
This IL-6 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with GTX74037 as the detection reagent.
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Recombinant murine IL-6
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_368318
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
interleukin 6 , Il-6
Cellular Localization
Secreted
Background
This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Database
Research Area