GeneTex
United States (US)

IL6 antibody

GTX29770_2453_WB_20180221_18121410_912.jpg
GTX29770_11_ELISA_20180221_18121410_571.jpg
GTX29770_857_IHC-P_20180221_18121410_154.jpg

Cat No. GTX29770

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA, Neut, sELISA
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1 - 0.2 μg/mL
IHC-P Assay dependent
ELISA 0.5 μg/mL
Neut 0.3 - 0.5 μg/mL
sELISA 0.5 - 2.0 ug/ml

Note :

Neut
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-6 (0.5 ng/mL)

sELISA
Capture : GTX29770, Detection : Biotinylated Anti-Rat IL-6 antibody

Not tested in other applications.

Calculated MW

24 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Interleukin 6,Ilg6,Ifnb2,Il6

Cellular Localization

Secreted

Background

Interleukin-6 is a potent lymphoid cell growth factor that affects B-lymphocytes, T lymphocytes, and hybridoma cells. It will also affect cytotoxic T cells in combination with other factors such as IL-2 and gamma-interferon.

Database

Research Area

DATA IMAGES
GTX29770_2453_WB_20180221_18121410_912.jpg

GTX29770 WB Image

WB analysis of sequential diluted rat IL-6 under either reducing or non-reducing conditions using GTX29770 IL6 antibody.
Working concentration : 0.1 - 0.2 ug/ml

GTX29770_11_ELISA_20180221_18121410_571.jpg

GTX29770 ELISA Image

ELISA analysis of rat IL-6 recombinant protein(0.2 - 0.4 ng/well) using GTX29770 IL6 antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Rat IL-6 as a detection antibody.

GTX29770_857_IHC-P_20180221_18121410_154.jpg

GTX29770 IHC-P Image

IHC-P analysis of colchicine injected rat brain (cingulate cortex) tissue using GTX29770 IL6 antibody.
Working concentration : 1 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin

Package List Price ($)
$ 319