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IL6 antibody

Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX29770
Anti-IL6 antibody used in ELISA (ELISA). GTX29770
Anti-IL6 antibody used in Western Blot (WB). GTX29770

Cat. No. GTX29770

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

 Rat
Package
50 μg ($399)
See all IL6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.2 μg/mL
IHC-P Assay dependent
ELISA 0.5 μg/mL
Neutralizing/Inhibition 0.3 - 0.5 μg/mL
Sandwich ELISA 0.5 - 2.0 ug/ml

Note :

Neutralizing/Inhibition
To yield one-half maximal inhibition [ND₅₀] of the biological activity of Rat IL-6 (0.5 ng/mL)

Sandwich ELISA
Capture : GTX29770, Detection : Biotinylated Anti-Rat IL-6 antibody
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , ILg6 , Ifnb2

Cellular Localization

Secreted

Background

Interleukin-6 is a potent lymphoid cell growth factor that affects B-lymphocytes, T lymphocytes, and hybridoma cells. It will also affect cytotoxic T cells in combination with other factors such as IL-2 and gamma-interferon.

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in IHC (Paraffin sections) (IHC-P). GTX29770

GTX29770 IHC-P Image

IHC-P analysis of colchicine injected rat brain (cingulate cortex) tissue using GTX29770 IL6 antibody.
Working concentration : 1 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin

Anti-IL6 antibody used in ELISA (ELISA). GTX29770

GTX29770 ELISA Image

ELISA analysis of rat IL-6 recombinant protein(0.2 - 0.4 ng/well) using GTX29770 IL6 antibody(capture antibody) at 0.5 - 2.0 ug/ml and Biotinylated Anti-Rat IL-6 as a detection antibody.

Anti-IL6 antibody used in Western Blot (WB). GTX29770

GTX29770 WB Image

WB analysis of sequential diluted rat IL-6 under either reducing or non-reducing conditions using GTX29770 IL6 antibody.
Working concentration : 0.1 - 0.2 ug/ml

REFERENCE

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SDS
PBS.pdf
Package List Price ($)
$ 399