GeneTex
United States (US)

IL6 antibody

GTX31178_IHC_18121410_814.jpg

Cat No. GTX31178

Host Rabbit
Clonality Polyclonal
Application WB, IHC-Fr, ELISA, Neut
Reactivity Mouse
APPLICATION

Application Note

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-Fr: Use at a concentration of 0.5 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Mouse IL-6 (3 pg/ml), a concentration of 0.24-0.36 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.

Calculated MW

24 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Recombinant Murine IL-6

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX31178_IHC_18121410_814.jpg

GTX31178 IHC-Fr Image

IHC analysis of colchicine injected mouse brain (cingulate cortex) tissue using IL6 antibody at a concentration of 0.5 μg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.

REFERENCE
Package List Price ($)
$ 319