GeneTex

  • Country / Location Selection

United States (US)

IL6 antibody

Anti-IL6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX31178

Cat. No. GTX31178

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, ELISA, Neutralizing/Inhibition

Reactivity

 Mouse
Package
100 μg ($399)
See all IL6 products

APPLICATION

Application Note

We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 μg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-Fr: Use at a concentration of 0.5 μg/ml. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of Mouse IL-6 (3 pg/ml), a concentration of 0.24-0.36 μg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant Murine IL-6

Conjugation

Unconjugated

RRID

AB_2887603

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 6 , Il-6

Cellular Localization

Secreted

Background

This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-IL6 antibody used in IHC (Frozen sections) (IHC-Fr). GTX31178

GTX31178 IHC-Fr Image

IHC analysis of colchicine injected mouse brain (cingulate cortex) tissue using IL6 antibody at a concentration of 0.5 μg/ml. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.

REFERENCE

Application Reference

XM Li et al. Cell Rep 2024; 43 (2) : 113688 Histone lactylation inhibits RARγ expression in macrophages to promote colorectal tumorigenesis through activation of TRAF6-IL-6-STAT3 signaling
Application : WB
Reactivity : Mouse
Liang YC et al. Biomedicine (Taipei) 2017; 7 (3) : 18 Torenia concolor Lindley var. formosana Yamazaki extracts improve inflammatory response and lipid accumulation via PPARs activation.
Application : WB
Reactivity : Mouse
Ma B et al. Am J Cancer Res 2017; 7 (3) : 433-447 Gpr110 deficiency decelerates carcinogen-induced hepatocarcinogenesis via activation of the IL-6/STAT3 pathway.
Application : WB
Reactivity : Mouse
Chen MC et al. PLoS One 2015; 10 (11) : e0143594 Supplementation of Magnolol Attenuates Skeletal Muscle Atrophy in Bladder Cancer-Bearing Mice Undergoing Chemotherapy via Suppression of FoxO3 Activation and Induction of IGF-1.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for IL6 antibody (GTX31178). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399