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IL7 Receptor alpha antibody [A7R34] (PE-Cy7)

Anti-IL7 Receptor alpha antibody [A7R34] (PE-Cy7) used in Flow cytometry (FACS). GTX01473-10

Cat. No. GTX01473-10

Reference 7

Host

 Rat

Clonality

 Monoclonal

Clone Name

 A7R34

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human, Mouse
Package
100 μg ($299)
See all IL7 Receptor alpha products
PRODUCT

Summary

The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Phycoerythrin-Cyanine7 (PE-Cy7) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 7 receptor , CD127 , IL-7Ralpha

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL7 Receptor alpha antibody [A7R34] (PE-Cy7) used in Flow cytometry (FACS). GTX01473-10

GTX01473-10 FACS Image

FACS analysis of mouse C57Bl/6 splenocytes using GTX01473-10 IL7 Receptor alpha antibody [A7R34] (PE-Cy7).
Right panel : co-stained with IL7 Receptor alpha antibody [A7R34] (PE-Cy7) and Mouse CD3 antibody (FITC)
Left panel : co-stained with isotype control and Mouse CD3 antibody (FITC)
antibody amount : 0.5 μg (5 μl)

REFERENCE

Clone Reference

2018; STAT3 regulates cytotoxicity of human CD57+ CD4+ T cells in blood and lymphoid follicles.
Application : FACS
Reactivity : Human
2012; Activation of the Hippo pathway by CTLA-4 regulates the expression of Blimp-1 in the CD8+ T cell.
2011; In vivo administration of the recombinant IL-7/hepatocyte growth factor β hybrid cytokine efficiently restores thymopoiesis and naive T cell generation in lethally irradiated mice after syngeneic bone marrow transplantation.
2009; LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.
2006; Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 299