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IL7 Receptor alpha antibody [A7R34]

Cat. No. GTX01473

Reference 8

Host

 Rat

Clonality

 Monoclonal

Clone Name

 A7R34

Isotype

 IgG2a

Application

 IHC-Fr, FACS, IP, Activation, Depletion

Reactivity

 Human, Mouse
Package
100 μg ($169)
See all IL7 Receptor alpha products
PRODUCT

Summary

The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Activation Assay dependent
Depletion Assay dependent
Inhibition assay Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Purity

> 90% (determined by SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 7 receptor , CD127 , IL-7Ralpha

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

Application Reference

Su F et al. Vaccines (Basel) 2023; 11 (4) Early Oral Administration of Ginseng Stem-Leaf Saponins Enhances the Peyer's Patch-Dependent Maternal IgA Antibody Response to a PEDV Inactivated Vaccine in Mice, with Gut Microbiota Involvement.

Clone Reference

2018; STAT3 regulates cytotoxicity of human CD57+ CD4+ T cells in blood and lymphoid follicles.
Application : FACS
Reactivity : Human
2012; Activation of the Hippo pathway by CTLA-4 regulates the expression of Blimp-1 in the CD8+ T cell.
Application : FACS
Reactivity : Mouse
2011; In vivo administration of the recombinant IL-7/hepatocyte growth factor β hybrid cytokine efficiently restores thymopoiesis and naive T cell generation in lethally irradiated mice after syngeneic bone marrow transplantation.
Application : Activation
Reactivity : Mouse
2009; LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.
Application : IHC-Fr
Reactivity : Mouse
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 169