WB analysis of IL7-treated and untreated D1 whole cell lysate using GTX48714 IL7 Receptor alpha (phospho Tyr449) antibody.
Dilution : 1:1000

WB analysis of IL7-treated and untreated D1 whole cell lysate using GTX48714 IL7 Receptor alpha (phospho Tyr449) antibody. IP with anti-phoshpotyrosine antibody
Dilution : 1:10000

Immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4ºC. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.

Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4ºC. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.