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IL7 Receptor alpha (phospho Tyr449) antibody

Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in Western Blot (WB). GTX48714
Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in Western Blot (WB). GTX48714

Cat No. GTX48714

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10000
ELISA 1:5000 - 1:50000
IHC Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.

Purification

Affinity purified using phosphopeptide and cross-adsorbed against non-phosphorylated peptide

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 7 receptor , CD127 , IL-7Ralpha

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Database

Research Area

DATA IMAGES

Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in Western Blot (WB). GTX48714

GTX48714 WB Image

Immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4ºC. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.

Anti-IL7 Receptor alpha (phospho Tyr449) antibody used in Western Blot (WB). GTX48714

GTX48714 WB Image

Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody (GTX48714). Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4ºC. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4ºC. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 289