WB analysis of recombinant human IL7 protein using GTX48673 IL7 antibody (Biotin).
Loading : 50 ng
Dilution : 1:1000

WB analysis of recombinant human IL7 protein using GTX48673 IL7 antibody (Biotin).
Dilution : 1:1000

Anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (GTX27090) secondary antibody diluted 1:40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate.