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IL9 antibody (Biotin)

Anti-IL9 antibody (Biotin) used in Western Blot (WB). GTX48670

Cat No. GTX48670

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, IHC

Reactivity

 Human
Package
50 μg ($289)
See all IL9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000 - 1:10,000
ELISA 1:20,000 - 1:100,000
IHC 1:1,000 - 1:5,000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Specificity/Sensitivity

In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids.

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide and 10 mg/mL Bovine Serum Albumin (BSA)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-9 protein.

Purification

IgG fraction

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 9 , HP40 , IL-9 , P40

Cellular Localization

Secreted

Background

IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.

Database

Research Area

DATA IMAGES

Anti-IL9 antibody (Biotin) used in Western Blot (WB). GTX48670

GTX48670 WB Image

Western Blot of Rabbit anti-Human IL-9 Biotin Conjugated Antibody. Load: 50 ng Human IL-9 per lane. Primary antibody: Human IL-9 primary antibody at 1:1,000 overnight at 4ºC. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 14 kDa for Human IL-9. Other band(s): None.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 289