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ILF2 antibody [6F1]

Overexpression
Anti-ILF2 antibody [6F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84290
Anti-ILF2 antibody [6F1] used in Western Blot (WB). GTX84290
Anti-ILF2 antibody [6F1] used in Western Blot (WB). GTX84290
Anti-ILF2 antibody [6F1] used in IHC (Paraffin sections) (IHC-P). GTX84290
Anti-ILF2 antibody [6F1] used in Western Blot (WB). GTX84290

Cat. No. GTX84290

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all ILF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human ILF2 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin enhancer binding factor 2 , NF45 , PRO3063

Cellular Localization

Cytoplasm,Nucleus,Nucleolus

Background

Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.

Database

DATA IMAGES

Anti-ILF2 antibody [6F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84290

GTX84290 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ILF2 plasmid using GTX84290 ILF2 antibody [6F1].

Anti-ILF2 antibody [6F1] used in Western Blot (WB). GTX84290

GTX84290 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84290 ILF2 antibody [6F1].
Loading : 10 ug per lane
Dilution : 1:500

Anti-ILF2 antibody [6F1] used in Western Blot (WB). GTX84290

GTX84290 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ILF2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84290 ILF2 antibody [6F1].
Loading : 5 ug per lane

Anti-ILF2 antibody [6F1] used in IHC (Paraffin sections) (IHC-P). GTX84290

GTX84290 IHC-P Image

IHC-P analysis of kidney tissue using GTX84290 ILF2 antibody [6F1].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ILF2 antibody [6F1] used in Western Blot (WB). GTX84290

GTX84290 WB Image

WB analysis of various cell lines using GTX84290 ILF2 antibody [6F1].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519