APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-2.5 ug/ml |
| 2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a C-terminal region of Human ILF2
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin enhancer binding factor 2 , NF45 , PRO3063
Cellular Localization
Cytoplasm,Nucleus,Nucleolus
Background
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
Database
DATA IMAGES
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GTX49071 IHC-P Image
IHC-P analysis of human kidney tissue using GTX49071 ILF2 antibody at 4.0-8.0μg/ml.
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GTX49071 WB Image
WB analysis of Jurkat cells using GTX49071 ILF2 antibody at 2.5μg/ml.
Lane A : Primary antibody
Lane B : Primary antibody + blocking peptide
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GTX49071 IHC-P Image
IHC-P analysis of human heart tissue using GTX49071 ILF2 antibody at 4.0-8.0μg/ml.
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GTX49071 WB Image
WB analysis of Jurkat cells using GTX49071 ILF2 antibody at 1.25μg/ml.
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REFERENCE
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REVIEW
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