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ILF2 antibody [JU50-33]

Recombinant antibody
Anti-ILF2 antibody [JU50-33] used in Western Blot (WB). GTX00979
Anti-ILF2 antibody [JU50-33] used in Western Blot (WB). GTX00979
Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979
Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979
Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979
Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979
Anti-ILF2 antibody [JU50-33] used in Flow cytometry (FACS). GTX00979
Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979

Cat No. GTX00979

Host

Rabbit

Clonality

Monoclonal

Clone Name

JU50-33

Isotype

IgG

Application

WB, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin enhancer binding factor 2 , ILF2 , NF45 , PRO3063

Cellular Localization

Nucleus,Nucleolus,Cytoplasm

Background

The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]

Database

DATA IMAGES

Anti-ILF2 antibody [JU50-33] used in Western Blot (WB). GTX00979

GTX00979 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ILF2 antibody [JU50-33] (GTX00979) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ILF2 antibody [JU50-33] used in Western Blot (WB). GTX00979

GTX00979 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILF2 antibody [JU50-33] (GTX00979) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979

GTX00979 IHC-P Image

IHC-P analysis of human tonsil tissue section using GTX00979 ILF2 antibody [JU50-33].

Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979

GTX00979 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00979 ILF2 antibody [JU50-33].

Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979

GTX00979 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00979 ILF2 antibody [JU50-33].

Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979

GTX00979 IHC-P Image

IHC-P analysis of human kidney tissue section using GTX00979 ILF2 antibody [JU50-33].

Anti-ILF2 antibody [JU50-33] used in Flow cytometry (FACS). GTX00979

GTX00979 FACS Image

FACS analysis of SH-SY-5Y cells using GTX00979 ILF2 antibody [JU50-33].
Red : primary antibody
Black : unlabelled control
Dilution : 1:100

Anti-ILF2 antibody [JU50-33] used in IHC (Paraffin sections) (IHC-P). GTX00979

GTX00979 IHC-P Image

IHC-P analysis of human liver cancer tissue section using GTX00979 ILF2 antibody [JU50-33].

REFERENCE

There are currently no references for ILF2 antibody [JU50-33] (GTX00979). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for ILF2 antibody [JU50-33] (GTX00979). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369