APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
Observed MW
90|110 kDa. The observed M.W. is based on the following publication. PMID: 33043604
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.85 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human ILF3
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin enhancer binding factor 3 , CBTF , DRBF , DRBP76 , MMP4 , MPHOSPH4 , MPP4 , NF-AT-90 , NF110 , NF110b , NF90 , NF90a , NF90b , NFAR , NFAR-1 , NFAR2 , TCP110 , TCP80
Cellular Localization
Cytoplasm , Nucleus , Nucleolus
Background
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/templates/default/js/lightbox/images/loading.gif "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
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GTX02844 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ILF3 antibody [GT1247] (GTX02844) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 33043604
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GTX02844 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ILF3 antibody [GT1247] (GTX02844) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 33043604
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GTX02844 IHC-P Image
IHC-P analysis of human placenta tissue section using GTX02844 ILF3 antibody [GT1247].
Dilution : 1:100
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GTX02844 IHC-P Image
IHC-P analysis of rat testis tissue section using GTX02844 ILF3 antibody [GT1247].
Dilution : 1:100
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GTX02844 WB Image
WB analysis of various samples using GTX02844 ILF3 antibody [GT1247].
Dilution : 1:3000
Loading : 25μg
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GTX02844 IHC-P Image
IHC-P analysis of mouse kidney tissue section using GTX02844 ILF3 antibody [GT1247].
Dilution : 1:100
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REFERENCE
There are currently no references for ILF3 antibody [GT1247] (GTX02844). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ILF3 antibody [GT1247] (GTX02844). Be the first to share your experience with this product.
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/upload/website/prouct_img/small/GTX02844/GTX02844_4000001438_20210122_WB_2_w_23053123_865.webp "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/upload/website/prouct_img/small/GTX02844/GTX02844_4000001438_20210122_WB_w_23053123_447.webp "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
![Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844](/upload/website/prouct_img/small/GTX02844/A8186_IHC_02_(1073002)_w_23053123_639.webp "Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844")
![Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844](/upload/website/prouct_img/small/GTX02844/A8186_IHC_01_(1073001)_w_23053123_429.webp "Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844")
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/upload/website/prouct_img/small/GTX02844/CutImage_A8186_WB_01_(1071176)_w_23053123_584.webp "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
![Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844](/upload/website/prouct_img/small/GTX02844/A8186_IHC_03_(1073003)_w_23053123_814.webp "Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844")
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/upload/website/prouct_img/normal/GTX02844/GTX02844_4000001438_20210122_WB_2_w_23053123_865.webp "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/upload/website/prouct_img/normal/GTX02844/GTX02844_4000001438_20210122_WB_w_23053123_447.webp "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
![Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844](/upload/website/prouct_img/normal/GTX02844/A8186_IHC_02_(1073002)_w_23053123_639.webp "Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844")
![Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844](/upload/website/prouct_img/normal/GTX02844/A8186_IHC_01_(1073001)_w_23053123_429.webp "Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844")
![Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844](/upload/website/prouct_img/normal/GTX02844/CutImage_A8186_WB_01_(1071176)_w_23053123_584.webp "Anti-ILF3 antibody [GT1247] used in Western Blot (WB). GTX02844")
![Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844](/upload/website/prouct_img/normal/GTX02844/A8186_IHC_03_(1073003)_w_23053123_814.webp "Anti-ILF3 antibody [GT1247] used in IHC (Paraffin sections) (IHC-P). GTX02844")