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ILK antibody [N1C1]

KO/KD
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691
Anti-ILK antibody [N1C1] used in Immunohistochemistry (IHC). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691
Anti-ILK antibody [N1C1] used in Immunoprecipitation (IP). GTX101691
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

Cat. No. GTX101691

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm, IP, IHC

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all ILK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
IHC Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A431 , Neuro2A , C8D30 , NIH-3T3 , C2Cl2 , PC-12 , Neuro 2A , C8D30 , Raw264.7 , HeLa

Predict Reactivity

Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

integrin linked kinase , HEL-S-28 , ILK-1 , ILK-2 , P59 , p59ILK

Cellular Localization

Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

KO/KD

Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ILK antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX101691

GTX101691 IHC-Wm Image

Immunofluorescent image of a pharyngeal cartilage section of a zebrafish embryo using ILK antibody [N1C1] (GTX101691) at a 1:200 dilution. ILK (Green)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish muscle
10% SDS PAGE
GTX101691 diluted at 1:1000

Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691

GTX101691 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ILK antibody [N1C1] (GTX101691) at 1:300 dilution.

Anti-ILK antibody [N1C1] used in Immunohistochemistry (IHC). GTX101691

GTX101691 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ILK antibody [N1C1] (GTX101691) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

Anti-ILK antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101691

GTX101691 IHC-P Image

ILK antibody [N1C1] detects ILK protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ILK antibody [N1C1] (GTX101691) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

ILK antibody [N1C1] detects ILK protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
10 % SDS-PAGE
ILK antibody [N1C1] (GTX101691) dilution: 1:1000

Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX101691 diluted at 1:500

Anti-ILK antibody [N1C1] used in Immunoprecipitation (IP). GTX101691

GTX101691 IP Image

ILK antibody [N1C1] immunoprecipitates ILK protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of ILK protein by 4 μg ILK antibody [N1C1] (GTX101691)
10 % SDS-PAGE
The immunoprecipitated ILK protein was detected by ILK antibody [N1C1] (GTX101691) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ILK antibody [N1C1] used in Western Blot (WB). GTX101691

GTX101691 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

REFERENCE

Application Reference

Murase H et al. Oncol Rep 2023; 50 (3)
H Murase et al. Oncology Reports 2023; 50 : 164
Application : WB
Chen YL et al. Sci Rep 2021; 11 (1) : 644 Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells.
Application : WB
Reactivity : Human
Wantoch von Rekowski K et al. Biomolecules 2019; 9 (12)
Application : WB
Reactivity : Human
Yen YC et al. Oncotarget 2015; 6 (39) : 41837-55 *-like growth factor-independent *-like growth factor binding protein 3 promotes cell migration and lymph node metastasis of oral squamous cell carcinoma cells by requirement of integrin β1.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169