GeneTex
United States (US)

ILK antibody [N1C1]

KO/KD
GTX101691_39876_20170330_WB_KO_watermark_19011506_927.jpg
GTX101691_39876_20160107_WB_M_19011506_227.jpg
GTX101691_39876_WB_M_19011506_889.jpg
GTX101691_WB_19011506_194.jpg
GTX101691_39876_IP_19011506_176.jpg
GTX101691_39876_20160107_WB_R_19011506_339.jpg
GTX101691_39876_20160113_IHC-P_M_19011506_939.jpg

Cat No. GTX101691

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

HeLa , A431 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Guinea pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ILK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686F1765 antibody, ILK-2 antibody, P59 antibody, ILK antibody, integrin-linked kinase-2 antibody, p59ILK antibody, 59 kDa serine/threonine-protein kinase antibody, ILK-1 antibody, integrin-linked protein kinase antibody, integrin-linked kinase antibody

Cellular Localization

Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX101691_39876_20170330_WB_KO_watermark_19011506_927.jpg

GTX101691 WB Image

KO/KD

Wild-type (WT) and ILK knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101691_39876_20160107_WB_M_19011506_227.jpg

GTX101691 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

GTX101691_39876_WB_M_19011506_889.jpg

GTX101691 WB Image

ILK antibody [N1C1] detects ILK protein by western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
10 % SDS-PAGE
ILK antibody [N1C1] (GTX101691) dilution: 1:1000

GTX101691_WB_19011506_194.jpg

GTX101691 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX101691 diluted at 1:500

GTX101691_39876_IP_19011506_176.jpg

GTX101691 IP Image

ILK antibody [N1C1] immunoprecipitates ILK protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of ILK protein by 4 μg ILK antibody [N1C1] (GTX101691)
10 % SDS-PAGE
The immunoprecipitated ILK protein was detected by ILK antibody [N1C1] (GTX101691) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX101691_39876_20160107_WB_R_19011506_339.jpg

GTX101691 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [N1C1] (GTX101691) diluted at 1:500.

GTX101691_39876_20160113_IHC-P_M_19011506_939.jpg

GTX101691 IHC-P Image

ILK antibody [N1C1] detects ILK protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ILK antibody [N1C1] (GTX101691) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reference
SDS
Glycerol.pdf
Thimerosal.pdf