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ILK antibody [SC68-04]

Recombinant antibody
Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046
Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046
Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046
Anti-ILK antibody [SC68-04] used in IHC (Paraffin sections) (IHC-P). GTX01046
Anti-ILK antibody [SC68-04] used in Flow cytometry (FACS). GTX01046

Cat No. GTX01046

Host

Rabbit

Clonality

Monoclonal

Clone Name

SC68-04

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

integrin linked kinase,HELS28,ILK,ILK1,ILK2,P59,p59ILK

Cellular Localization

Cell junction,Focal adhesion,Cell membrane,Cell projection,Lamellipodium,Cytoplasm,Myofibril,Sarcomere

Background

This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Database

Research Area

DATA IMAGES

Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046

GTX01046 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [SC68-04] (GTX01046) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046

GTX01046 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX01046 ILK antibody [SC68-04].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046

GTX01046 ICC/IF Image

ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX01046 ILK antibody [SC68-04].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-ILK antibody [SC68-04] used in IHC (Paraffin sections) (IHC-P). GTX01046

GTX01046 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX01046 ILK antibody [SC68-04].

Anti-ILK antibody [SC68-04] used in Flow cytometry (FACS). GTX01046

GTX01046 FACS Image

FACS analysis of Jurkat cells using GTX01046 ILK antibody [SC68-04].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

REFERENCE

There are currently no references for ILK antibody [SC68-04] (GTX01046). Be the first to share your publications with this product.

REVIEW

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