APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:2000 |
| 1:50-1:200 |
| 1:50-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
integrin linked kinase , HELS28 , ILK , ILK1 , ILK2 , P59 , p59ILK
Cellular Localization
Cell junction,Focal adhesion,Cell membrane,Cell projection,Lamellipodium,Cytoplasm,Myofibril,Sarcomere
Background
This gene encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Database
Research Area
DATA IMAGES
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/templates/default/js/lightbox/images/loading.gif "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
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GTX01046 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [SC68-04] (GTX01046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01046 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [SC68-04] (GTX01046) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01046 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ILK antibody [SC68-04] (GTX01046) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01046 ICC/IF Image
ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX01046 ILK antibody [SC68-04].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01046 ICC/IF Image
ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX01046 ILK antibody [SC68-04].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS
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GTX01046 IHC-P Image
IHC-P analysis of rat brain tissue section using GTX01046 ILK antibody [SC68-04].
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GTX01046 FACS Image
FACS analysis of Jurkat cells using GTX01046 ILK antibody [SC68-04].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50
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REFERENCE
There are currently no references for ILK antibody [SC68-04] (GTX01046). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ILK antibody [SC68-04] (GTX01046). Be the first to share your experience with this product.
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_HJ0629_20200807_WB_M_w_23053121_629.webp "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_HJ0629_20200807_WB_R_w_23053121_283.webp "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_HJ0629_20200214_WB_w_23053121_261.webp "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
![Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_20200303_ICC-IF_300_w_23053121_186.webp "Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046")
![Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_20200303_ICC-IF_299_w_23053121_369.webp "Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046")
![Anti-ILK antibody [SC68-04] used in IHC (Paraffin sections) (IHC-P). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_20200303_IHC-P_301_w_23053121_729.webp "Anti-ILK antibody [SC68-04] used in IHC (Paraffin sections) (IHC-P). GTX01046")
![Anti-ILK antibody [SC68-04] used in Flow cytometry (FACS). GTX01046](/upload/website/prouct_img/small/GTX01046/GTX01046_20200303_FACS_302_w_23053121_875.webp "Anti-ILK antibody [SC68-04] used in Flow cytometry (FACS). GTX01046")
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_HJ0629_20200807_WB_M_w_23053121_629.webp "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_HJ0629_20200807_WB_R_w_23053121_283.webp "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
![Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_HJ0629_20200214_WB_w_23053121_261.webp "Anti-ILK antibody [SC68-04] used in Western Blot (WB). GTX01046")
![Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_20200303_ICC-IF_300_w_23053121_186.webp "Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046")
![Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_20200303_ICC-IF_299_w_23053121_369.webp "Anti-ILK antibody [SC68-04] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01046")
![Anti-ILK antibody [SC68-04] used in IHC (Paraffin sections) (IHC-P). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_20200303_IHC-P_301_w_23053121_729.webp "Anti-ILK antibody [SC68-04] used in IHC (Paraffin sections) (IHC-P). GTX01046")
![Anti-ILK antibody [SC68-04] used in Flow cytometry (FACS). GTX01046](/upload/website/prouct_img/normal/GTX01046/GTX01046_20200303_FACS_302_w_23053121_875.webp "Anti-ILK antibody [SC68-04] used in Flow cytometry (FACS). GTX01046")