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IMP3 antibody

Anti-IMP3 antibody used in Western Blot (WB). GTX115459
Anti-IMP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115459
Anti-IMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115459

Cat. No. GTX115459

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all IMP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

PC-3 , SK-N-SH

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165259

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 2 mRNA binding protein 3 , CT98 , IMP-3 , IMP3 , KOC , KOC1 , VICKZ3

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IMP3 antibody used in Western Blot (WB). GTX115459

GTX115459 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
B: SK-N-SH
7.5% SDS PAGE
GTX115459 diluted at 1:1000

Anti-IMP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115459

GTX115459 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IMP-3(GTX115459) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115459

GTX115459 ICC/IF Image

IMP3 antibody detects IMP3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IMP3 protein stained by IMP3 antibody (GTX115459) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169