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IMP3 antibody

Anti-IMP3 antibody used in Western Blot (WB). GTX115459
Anti-IMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115459
Anti-IMP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115459

Cat No. GTX115459

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

PC-3 , SK-N-SH

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

insulin like growth factor 2 mRNA binding protein 3,CT98,IMP-3,IMP3,KOC,KOC1,VICKZ3

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-IMP3 antibody used in Western Blot (WB). GTX115459

GTX115459 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
B: SK-N-SH
7.5% SDS PAGE
GTX115459 diluted at 1:1000

Anti-IMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115459

GTX115459 ICC/IF Image

IMP3 antibody detects IMP3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IMP3 protein stained by IMP3 antibody (GTX115459) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-IMP3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115459

GTX115459 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using IMP-3(GTX115459) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169