APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT2G5 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IMPA3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
inositol monophosphatase 1 , IMP , IMPA , IMPA1
Cellular Localization
Cytoplasm
Background
This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
![Anti-IMPA1 antibody [AT2G5] used in Flow cytometry (FACS). GTX57655](/templates/default/js/lightbox/images/loading.gif "Anti-IMPA1 antibody [AT2G5] used in Flow cytometry (FACS). GTX57655")
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GTX57655 FACS Image
FACS analysis of LNCap cells using GTX57655 IMPA1 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57655 ICC/IF Image
ICC/IF analysis of LNCap cells using GTX57655 IMPA1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57655 ICC/IF Image
ICC/IF analysis of Hep3B cells using GTX57655 IMPA1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57655 WB Image
WB analysis of various samples using GTX57655 IMPA1 antibody.
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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REFERENCE
There are currently no references for IMPA1 antibody [AT2G5] (GTX57655). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IMPA1 antibody [AT2G5] (GTX57655). Be the first to share your experience with this product.
![Anti-IMPA1 antibody [AT2G5] used in Flow cytometry (FACS). GTX57655](/upload/website/prouct_img/small/GTX57655/GTX57655_20200204_FC_w_23061123_709.webp "Anti-IMPA1 antibody [AT2G5] used in Flow cytometry (FACS). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655](/upload/website/prouct_img/small/GTX57655/GTX57655_20200204_IF2_w_23061123_111.webp "Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655](/upload/website/prouct_img/small/GTX57655/GTX57655_20200204_IF1_w_23061123_713.webp "Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Western Blot (WB). GTX57655](/upload/website/prouct_img/small/GTX57655/GTX57655_20200204_WB_w_23061123_103.webp "Anti-IMPA1 antibody [AT2G5] used in Western Blot (WB). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Flow cytometry (FACS). GTX57655](/upload/website/prouct_img/normal/GTX57655/GTX57655_20200204_FC_w_23061123_709.webp "Anti-IMPA1 antibody [AT2G5] used in Flow cytometry (FACS). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655](/upload/website/prouct_img/normal/GTX57655/GTX57655_20200204_IF2_w_23061123_111.webp "Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655](/upload/website/prouct_img/normal/GTX57655/GTX57655_20200204_IF1_w_23061123_713.webp "Anti-IMPA1 antibody [AT2G5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57655")
![Anti-IMPA1 antibody [AT2G5] used in Western Blot (WB). GTX57655](/upload/website/prouct_img/normal/GTX57655/GTX57655_20200204_WB_w_23061123_103.webp "Anti-IMPA1 antibody [AT2G5] used in Western Blot (WB). GTX57655")