APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/1000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human INCENP (AA: 369-583) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
inner centromere protein
Cellular Localization
Nucleus
Background
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]
Database
Research Area
DATA IMAGES
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GTX60619 FACS Image
FACS analysis of Jurkat cells using GTX60619 INCENP antibody [3D2]. Green : INCENP Red : negative control
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GTX60619 WB Image
WB analysis of HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate using GTX60619 INCENP antibody [3D2].
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GTX60619 IHC-P Image
IHC-P analysis of rectum cancer tissue using GTX60619 INCENP antibody [3D2].
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GTX60619 IHC-P Image
IHC-P analysis of cervical cancer tissue using GTX60619 INCENP antibody [3D2].
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GTX60619 ELISA Image
ELISA analysis of antigen using GTX60619 INCENP antibody [3D2].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60619 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX60619 INCENP antibody [3D2]. Green : INCENP Blue: DRAQ5 fluorescent DNA dye
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REFERENCE
There are currently no references for INCENP antibody [3D2] (GTX60619). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for INCENP antibody [3D2] (GTX60619). Be the first to share your experience with this product.