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INDOL1 antibody

Anti-INDOL1 antibody used in Western Blot (WB). GTX11947
Anti-INDOL1 antibody used in Flow cytometry (FACS). GTX11947

Cat. No. GTX11947

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
100 μg ($439)
See all INDOL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
FACS 1-3μg/1x10⁶cells
Not tested in other applications.

Calculated MW

47.08 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1% Na₂HPO₄, 0.45% NaCl, 2.5% BSA

Preservative

0.025% Thimerosal, 0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

500 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human INDOL1(1-20aa MLHFHYYDTSNKIMEPHRPN).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2885445

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011]

Database

DATA IMAGES

Anti-INDOL1 antibody used in Western Blot (WB). GTX11947

GTX11947 WB Image

WB analysis of various samples using GTX11947 INDOL1 antibody.
Lane 1 : human A549 whole cell lysates (positive control)
Lane 2 : human placenta tissue lysates (positive control)
Lane 3 : human Caco-2 whole cell lysates (positive control)
Lane 4 : human PC-3 whole cell lysates (negative control)
Dilution : 0.5 μg/mL
Loading : 50μg of sample under reducing conditions

Anti-INDOL1 antibody used in Flow cytometry (FACS). GTX11947

GTX11947 FACS Image

FACS analysis of SiHa cells using GTX11947 INDOL1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Cell only control
Antibody amount : 1μg/1x10⁶ cells for 30 min at 20ºC

REFERENCE

Application Reference

Wang LT et al. Cancer Res 2017; (Epub) Intestine-specific homeobox gene ISX integrates interleukin-6 signaling, tryptophan catabolism and immune suppression.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Thimerosal.pdf
Package List Price ($)
$ 439