WB analysis of recombinant human ING5 protein using GTX48484 ING5 antibody.
Loading : 10 μg
Dilution : 1:1500

WB analysis of transfected and non-transfected HeLa nuclear extracts using GTX48484 ING5 antibody.
Lane 1 : Mock
Lane 2 : ING4 overexpressed HeLa whole cell lysate
Lane 3 : ING5 overexpressed HeLa whole cell lysate
Dilution : 1:500

Western blot analysis is shown using GeneTex's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results.

Western blot analysis is shown using GeneTex's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 μg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500.Incubation was overnight at 4º C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.