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INPP5F antibody

Anti-INPP5F antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103107
Anti-INPP5F antibody used in IHC (Paraffin sections) (IHC-P). GTX103107
Anti-INPP5F antibody used in Western Blot (WB). GTX103107

Cat No. GTX103107

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

293T , A431

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human INPP5F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

OCRL inositol polyphosphate-5-phosphatase , INPP5F , LOCR , NPHL2 , OCRL-1 , OCRL1

Background

This gene encodes a phosphatase enzyme that is involved in actin polymerization and is found in the trans-Golgi network. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. [provided by RefSeq]

Database

DATA IMAGES
Anti-INPP5F antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103107

GTX103107 ICC/IF Image

OCRL antibody detects OCRL protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: OCRL protein stained by OCRL antibody (GTX103107) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-INPP5F antibody used in IHC (Paraffin sections) (IHC-P). GTX103107

GTX103107 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using OCRL(GTX103107) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-INPP5F antibody used in Western Blot (WB). GTX103107

GTX103107 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
5% SDS PAGE
GTX103107 diluted at 1:500

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169