GeneTex
United States (US)

INPP5F antibody

KO/KD
GTX104273_39904_WB_M_brain_19011506_817.jpg
GTX104273_WB_19011506_807.jpg
GTX104273_39904_IFA_19011506_808.jpg
GTX104273_39904_IHC_19011506_424.jpg
GTX104273_39904_IHC_M_19011506_410.jpg

Cat No. GTX104273

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

HeLa , mouse brain

Specificity/Sensitivity

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human INPP5F. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Inositol Polyphosphate-5-Phosphatase F,Mstp007,Mstpo47,Sac2,Hsac2,Inpp5F

Background

The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants have been observed, but most of them are not thought to be protein-coding. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104273_39904_WB_M_brain_19011506_817.jpg

GTX104273 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX104273 diluted at 1:500

GTX104273_WB_19011506_807.jpg

GTX104273 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
INPP5F antibody
GTX104273 diluted at 1:1000

GTX104273_39904_IFA_19011506_808.jpg

GTX104273 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using INPP5F(GTX104273) antibody at 1:200 dilution.

GTX104273_39904_IHC_19011506_424.jpg

GTX104273 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using INPP5F(GTX104273) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104273_39904_IHC_M_19011506_410.jpg

GTX104273 IHC-P Image

INPP5F antibody detects INPP5F protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
INPP5F antibody (GTX104273) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REVIEW

INPP5F antibody Cat No. GTX104273

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 22-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Ava5 cells
Blocking : 4% BSA, 1Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Permeabilization /Antigen retrieval : 0.2% Triton X-100, 0.3Hr
Primary Antibody : 1:500, 25°C, 1Hr
Figure Note : A: Ava5 cells, B: knockdown of INPP5F in Ava5 cells
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh-7 cells
Amount used : 20μg
Blocking : BlockPRO? Blocking buffer, 25°C, 3Hr
Primary Antibody : 1:3000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169