APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
human fetal spleen , human putamen , rat cortex
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to an Internal region of Human INSIG1
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
insulin induced gene 1 , CL6
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Database
Research Area
DATA IMAGES
|
GTX47438 WB Image
WB analysis of human putamen and rat cortex tissue using GTX47438 INSIG1 antibody at 1:1000.
Lane 1: human putamen lysate (50ug)
Lane 2: rat cortex lysate (50ug)
|
|
GTX47438 WB Image
WB analysis of human fetal spleen tissue using GTX47438 INSIG1 antibody at 0.2-1μg/ml.
|
|
GTX47438 IHC-P Image
IHC-P analysis of human liver tissue using GTX47438 INSIG1 antibody at 10μg/ml.
|
REFERENCE
There are currently no references for INSIG1 antibody, Internal (GTX47438). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for INSIG1 antibody, Internal (GTX47438). Be the first to share your experience with this product.