GeneTex
  • Country / Location Selection

United States (US)

INSIG1 antibody, Internal

Anti-INSIG1 antibody, Internal used in Western Blot (WB). GTX47438
Anti-INSIG1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX47438
Anti-INSIG1 antibody, Internal used in Western Blot (WB). GTX47438

Cat No. GTX47438

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
50 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

human fetal spleen , human putamen , rat cortex

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human INSIG1

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

insulin induced gene 1 , CL6

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-INSIG1 antibody, Internal used in Western Blot (WB). GTX47438

GTX47438 WB Image

WB analysis of human putamen and rat cortex tissue using GTX47438 INSIG1 antibody at 1:1000.
Lane 1: human putamen lysate (50ug)
Lane 2: rat cortex lysate (50ug)

Anti-INSIG1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX47438

GTX47438 IHC-P Image

IHC-P analysis of human liver tissue using GTX47438 INSIG1 antibody at 10μg/ml.

Anti-INSIG1 antibody, Internal used in Western Blot (WB). GTX47438

GTX47438 WB Image

WB analysis of human fetal spleen tissue using GTX47438 INSIG1 antibody at 0.2-1μg/ml.

REFERENCE

There are currently no references for INSIG1 antibody, Internal (GTX47438). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for INSIG1 antibody, Internal (GTX47438). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf