IP3 Receptor I antibody [HL1541]

Cat. No. GTX637015

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1541
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all IP3 Receptor I products

Datasheet

View product citations for antibody GTX637015 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

313 kDa. ( Note )

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IP3 Receptor I. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol 1,4,5-trisphosphate receptor 1 , D6Pas2 , Gm10429 , IP3R 1 , IP3R1 , InsP3R , Ip3r , Itpr-1 , P400 , Pcp-1 , Pcp1 , insP3R1 , opt , wblo

Cellular Localization

Endoplasmic reticulum membrane

Database

Gene ID: 16438 Itpr1
UniProt: P11881 Itpr1

Research Area

DATA IMAGES

GTX637015 IHC-P Image

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The expression pattern is based on the publication:PMID: 25409824

GTX637015 WB Image

Whole cell extract (30 Âμg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX637015 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637015 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637015 IHC-P Image

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637015 IHC-P Image

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637015 ICC/IF Image

IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637015 WB Image

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

IP3 Receptor I antibody [HL1541]

Cat. No. GTX637015

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX637015 IHC-P Image

GTX637015 WB Image

GTX637015 WB Image

GTX637015 WB Image

GTX637015 IHC-P Image

GTX637015 IHC-P Image

GTX637015 ICC/IF Image

GTX637015 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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