IP3 Receptor I antibody

Cat No. GTX133104

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

See all IP3 Receptor I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

313 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse IP3 Receptor I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886827

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inositol 1,4,5-trisphosphate receptor 1 , D6Pas2 , Gm10429 , IP3R 1 , IP3R1 , InsP3R , Ip3r , Itpr-1 , P400 , Pcp-1 , Pcp1 , insP3R1 , opt , wblo

Database

Gene ID: 16438 Itpr1
UniProt: P11881 Itpr1

Research Area

DATA IMAGES

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Itpr1 protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Blue: Hoechst 33342 staining.

GTX133104 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody (GTX133104) diluted at 1:500.

GTX133104 IHC-Fr Image

IP3 Receptor I antibody detects IP3 Receptor I protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: IP3 Receptor I protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody (GTX133104) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).