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IP3 Receptor I antibody

Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104
Anti-IP3 Receptor I antibody used in IHC (Frozen sections) (IHC-Fr). GTX133104
Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104

Cat. No. GTX133104

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IP3 Receptor I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

313 kDa. ( Note )

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse IP3 Receptor I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886827

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

inositol 1,4,5-trisphosphate receptor 1 , D6Pas2 , Gm10429 , IP3R 1 , IP3R1 , InsP3R , Ip3r , Itpr-1 , P400 , Pcp-1 , Pcp1 , insP3R1 , opt , wblo

Database

Research Area

DATA IMAGES

Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Itpr1 protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Blue: Hoechst 33342 staining.
Anti-IP3 Receptor I antibody used in IHC (Frozen sections) (IHC-Fr). GTX133104

GTX133104 IHC-Fr Image

IP3 Receptor I antibody detects IP3 Receptor I protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: IP3 Receptor I protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody (GTX133104) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Yu T et al. Exp Cell Res 2021; 399 (2) : 112438 Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes.
Luo X et al. EBioMedicine 2020; 56 : 102797 Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169