GeneTex
United States (US)

IP3 Receptor I antibody

GTX133104_42557_20160817_IFA_18121410_863.jpg
GTX133104_42557_20170105_WB_M_R_18121410_735.jpg
GTX133104_42557_20170608_IHC-P_M_18121410_796.jpg
GTX133104_42557_20171115_ICC_IF_R_18121410_876.jpg

Cat No. GTX133104

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

313 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse ITPR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

D6Pas2 antibody , ENSMUSG00000072853 antibody , Gm10429 antibody , IP3R1 antibody , ITPR1 antibody , ITPR1 antibody , InsP3R antibody , Ip3r antibody , P400 antibody , Pcp1 antibody , inositol 1,4,5-trisphosphate receptor 1 antibody , opt antibody

Database

Research Area

DATA IMAGES
GTX133104_42557_20160817_IFA_18121410_863.jpg

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Itpr1 protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Blue: Hoechst 33342 staining.

GTX133104_42557_20170105_WB_M_R_18121410_735.jpg

GTX133104 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody (GTX133104) diluted at 1:500.

GTX133104_42557_20170608_IHC-P_M_18121410_796.jpg

GTX133104 IHC-Fr Image

IP3 Receptor I antibody detects IP3 Receptor I protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: IP3 Receptor I protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133104_42557_20171115_ICC_IF_R_18121410_876.jpg

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody (GTX133104) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf