IP3 Receptor I antibody

Cat No. GTX133104

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr
Reactivity	Human, Mouse, Rat

See all IP3 Receptor I products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

313 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse ITPR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Inositol 1,4,5-Trisphosphate Receptor 1,D6Pas2,Gm10429,Ip3R 1,Ip3R1,Insp3R,Ip3R,Itpr-1,P400,Pcp-1,Pcp1,Insp3R1,Opt,Wblo,Itpr1

Database

Gene ID: 16438
UniProt: P11881

Research Area

DATA IMAGES

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Itpr1 protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Blue: Hoechst 33342 staining.

GTX133104 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody (GTX133104) diluted at 1:500.

GTX133104 IHC-Fr Image

IP3 Receptor I antibody detects IP3 Receptor I protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: IP3 Receptor I protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody (GTX133104) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).