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IP3 Receptor I antibody

Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104
Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104
Anti-IP3 Receptor I antibody used in IHC (Frozen sections) (IHC-Fr). GTX133104

Cat. No. GTX133104

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IP3 Receptor I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

313 kDa. ( Note )

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse IP3 Receptor I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886827

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol 1,4,5-trisphosphate receptor 1 , D6Pas2 , Gm10429 , IP3R 1 , IP3R1 , InsP3R , Ip3r , Itpr-1 , P400 , Pcp-1 , Pcp1 , insP3R1 , opt , wblo

Database

Research Area

DATA IMAGES

Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Itpr1 protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Blue: Hoechst 33342 staining.
Anti-IP3 Receptor I antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133104

GTX133104 ICC/IF Image

IP3 Receptor I antibody detects IP3 Receptor I protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3 Receptor I stained by IP3 Receptor I antibody (GTX133104) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IP3 Receptor I antibody used in IHC (Frozen sections) (IHC-Fr). GTX133104

GTX133104 IHC-Fr Image

IP3 Receptor I antibody detects IP3 Receptor I protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: IP3 Receptor I protein stained by IP3 Receptor I antibody (GTX133104) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Yu T et al. Exp Cell Res 2021; 399 (2) : 112438 Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes.
Luo X et al. EBioMedicine 2020; 56 : 102797 Obesity induces preadipocyte CD36 expression promoting inflammation via the disruption of lysosomal calcium homeostasis and lysosome function.
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169