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IP3KC antibody [N3C2], Internal

Anti-IP3KC antibody [N3C2], Internal used in Western Blot (WB). GTX104192
Anti-IP3KC antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104192

Cat. No. GTX104192

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IP3KC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IP3KC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177831

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol-trisphosphate 3-kinase C , IP3-3KC , IP3KC

Cellular Localization

Nucleus , Cytoplasm

Background

Inositol 1,4,5-trisphosphate 3-kinase (ITPK) catalyzes the phosphorylation of Ins(1,4,5)P3 to Ins(1,3,4,5)P4, both of which are modulators of calcium homeostasis. ITPK isoforms include ITPKA (MIM 147521), ITPKB (MIM 147522), and ITPKC, all of which contain a conserved catalytic unit in their C termini, but have unique N-terminal sequences and tissue distributions.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IP3KC antibody [N3C2], Internal used in Western Blot (WB). GTX104192

GTX104192 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX104192 diluted at 1:1000

Anti-IP3KC antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104192

GTX104192 ICC/IF Image

IP3KC antibody [N3C2], Internal detects IP3KC protein at nucleus by immunofluorescent analysis.
Sample: RD cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IP3KC protein stained by IP3KC antibody [N3C2], Internal (GTX104192) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169