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IPMK antibody [N1C2]

KO/KD Overexpression
Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954
Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954
Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954
Anti-IPMK antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104954
Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954
Anti-IPMK antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104954
Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954

Cat. No. GTX104954

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IPMK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IPMK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inositol polyphosphate multikinase

Cellular Localization

Nucleus

Background

This gene encodes a member of the inositol phosphokinase family. The encoded protein has 3-kinase, 5-kinase and 6-kinase activities on phosphorylated inositol substrates. The encoded protein plays an important role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate, and has a preferred 5-kinase activity. This gene may play a role in nuclear mRNA export. Pseudogenes of this gene are located on the long arm of chromosome 13 and the short arm of chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954

GTX104954 WB Image

KO/KD

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (GTX104954) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954

GTX104954 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104954 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954

GTX104954 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (GTX104954) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IPMK antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104954

GTX104954 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IPMK(GTX104954) antibody at 1:200 dilution.

Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954

GTX104954 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (GTX104954) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IPMK antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX104954

GTX104954 IHC-P Image

IPMK antibody [N1C2] detects IPMK protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
IPMK stained by IPMK antibody [N1C2] (GTX104954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IPMK antibody [N1C2] used in Western Blot (WB). GTX104954

GTX104954 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (GTX104954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ahmed I et al. Proc Natl Acad Sci U S A 2015; 112 (31) : 2867672 Huntington's disease: Neural dysfunction linked to inositol polyphosphate multikinase.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169